| Accession | GenProp0791 |
| Name | coenzyme F420 biosynthesis |
| Type | METAPATH |
| Description | The chromophore of coenzyme F420 is 7,8-didemethyl-8-hydroxy-5-deazaflavin, or FO, which is also used in a cyanobacterial DNA photoreactivating enzyme. Modification of FO to create coenzyme F420 includes the GTP-dependent addition of two glutamate residues in Methanococcus jannaschii and five or six in Mycobacterium bovis. CofF, which caps the gamma-glutamyl tail after two glutamates, occurs only the archaea. |
| JCVI Role | Other |
| Parent Property | GenProp0184: cofactor biosynthesis
|
| Literature References | [ 1 ] Grochowski LL, Xu H, White RH Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis. AB - Coenzyme F 420 is a hydride carrier cofactor functioning in methanogenesis. One step in the biosynthesis of coenzyme F 420 inv Biochemistry. 2008 Feb 9 PMID 18260642
[ 2 ] Graupner M, Xu H, White RH Characterization of the 2-phospho-L-lactate transferase enzyme involved in coenzyme F(420) biosynthesis in Methanococcus jannaschii. Biochemistry. 2002 Mar 19;41(11):3754-61. PMID 11888293
[ 3 ] Li H, Graupner M, Xu H, White RH CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii. Biochemistry. 2003 Aug 19;42(32):9771-8. PMID 12911320
[ 4 ] Choi KP, Bair TB, Bae YM, Daniels L Use of transposon Tn5367 mutagenesis and a nitroimidazopyran-based selection system to demonstrate a requirement for fbiA and fbiB in coenzyme F(420) biosynthesis by Mycobacterium bovis BCG. J Bacteriol. 2001 Dec;183(24):7058-66. PMID 11717263
|
|
| Gene Ontology Term | GO:0009108: coenzyme biosynthetic process (biological_process)
|
| Step Name | Step Num | Required | Evidence (Method) | Evidence Go Terms |
|---|
| lactoylphosphate guanylyltransferase | 3_CofC | YES | TIGR03552 (HMM): 2-phospho-L-lactate guanylyltransferase | GO:0009108: coenzyme biosynthetic process
|
| LPPG:Fo 2-phospho-L-lactate transferase | 4_CofD | YES | TIGR01819 (HMM): 2-phospho-L-lactate transferase | GO:0009108: coenzyme biosynthetic process
|
| F420-O:gamma-glutamyl ligase | 5_CofE | YES | TIGR01916 (HMM): coenzyme F420-0:L-glutamate ligase | GO:0009108: coenzyme biosynthetic process
|
| coenzyme gamma-F420-2:alpha-L-glutamate ligase | 6_CofF | NO | TIGR04443 (HMM): coenzyme gamma-F420-2:alpha-L-glutamate ligase | GO:0009108: coenzyme biosynthetic process
|
| FO biosynthesis | FO_syn | YES | GenProp0792 (GENPROP): 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) biosynthesis
| GO:0009108: coenzyme biosynthetic process
|
| F420 biosynthesis protein FbiB, C-terminal domain | FbiB_C | NO | TIGR03553 (HMM): F420 biosynthesis protein FbiB, C-terminal domain | GO:0009108: coenzyme biosynthetic process
|
| NADPH-dependent F420 reductase | reduct | NO | TIGR01915 (HMM): NADPH-dependent F420 reductase | |
