Genome Property Definition Page

Namecoenzyme F420 biosynthesis
DescriptionThe chromophore of coenzyme F420 is 7,8-didemethyl-8-hydroxy-5-deazaflavin, or FO, which is also used in a cyanobacterial DNA photoreactivating enzyme. Modification of FO to create coenzyme F420 includes the GTP-dependent addition of two glutamate residues in Methanococcus jannaschii and five or six in Mycobacterium bovis. CofF, which caps the gamma-glutamyl tail after two glutamates, occurs only the archaea.
JCVI RoleOther
Parent PropertyGenProp0184: cofactor biosynthesis
Literature References
[ 1 ] Grochowski LL, Xu H, White RH   Identification and Characterization of the 2-Phospho-l-lactate Guanylyltransferase Involved in Coenzyme F420 Biosynthesis. AB - Coenzyme F 420 is a hydride carrier cofactor functioning in methanogenesis. One step in the biosynthesis of coenzyme F 420 inv   Biochemistry. 2008 Feb 9  PMID 18260642
[ 2 ] Graupner M, Xu H, White RH   Characterization of the 2-phospho-L-lactate transferase enzyme involved in coenzyme F(420) biosynthesis in Methanococcus jannaschii.   Biochemistry. 2002 Mar 19;41(11):3754-61.  PMID 11888293
[ 3 ] Li H, Graupner M, Xu H, White RH   CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii.   Biochemistry. 2003 Aug 19;42(32):9771-8.  PMID 12911320
[ 4 ] Choi KP, Bair TB, Bae YM, Daniels L   Use of transposon Tn5367 mutagenesis and a nitroimidazopyran-based selection system to demonstrate a requirement for fbiA and fbiB in coenzyme F(420) biosynthesis by Mycobacterium bovis BCG.   J Bacteriol. 2001 Dec;183(24):7058-66.  PMID 11717263
Gene Ontology TermGO:0009108: coenzyme biosynthetic process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
lactoylphosphate guanylyltransferase3_CofCYESTIGR03552 (HMM): 2-phospho-L-lactate guanylyltransferaseGO:0009108: coenzyme biosynthetic process
LPPG:Fo 2-phospho-L-lactate transferase4_CofDYESTIGR01819 (HMM): 2-phospho-L-lactate transferaseGO:0009108: coenzyme biosynthetic process
F420-O:gamma-glutamyl ligase5_CofEYESTIGR01916 (HMM): coenzyme F420-0:L-glutamate ligaseGO:0009108: coenzyme biosynthetic process
coenzyme gamma-F420-2:alpha-L-glutamate ligase6_CofFNOTIGR04443 (HMM): coenzyme gamma-F420-2:alpha-L-glutamate ligaseGO:0009108: coenzyme biosynthetic process
FO biosynthesisFO_synYESGenProp0792 (GENPROP): 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) biosynthesis
GO:0009108: coenzyme biosynthetic process
F420 biosynthesis protein FbiB, C-terminal domainFbiB_CNOTIGR03553 (HMM): F420 biosynthesis protein FbiB, C-terminal domainGO:0009108: coenzyme biosynthetic process
NADPH-dependent F420 reductasereductNOTIGR01915 (HMM): NADPH-dependent F420 reductase

Parent Properties
GenProp0184cofactor biosynthesis

Sibling Properties
GenProp0001chorismate biosynthesis via shikimate
GenProp0030glutathione biosynthesis
GenProp0036biotin biosynthesis
GenProp0038tetrahydrofolate biosynthesis from GTP and PABA
GenProp0057NAD(P) biosynthesis from L-aspartate and DHAP
GenProp0112riboflavin, FAD and FMN from GTP and ribulose-5-P
GenProp0124pantothenate biosynthesis from aspartate and 2-oxoisovalerate
GenProp0136ubiquinone biosynthesis from chorismate, aerobic
GenProp0140iron-sulfur cluster assembly systems
GenProp0170coenzyme PQQ biosynthesis
GenProp0171coenzyme A biosynthesis from pantothenate
GenProp0194siroheme biosynthesis from uroporphrinogen-III
GenProp0220uroporhyrinogen III biosynthesis from 5-aminolevulinate
GenProp0221protoporphyrin IX biosynthesis from uroporphyrinogen III
GenProp0222protoheme from protoporphyrin IX
GenProp0250thiamine: hydroxyethylthiazole from 1-deoxy-D-xylulose-5-phosphate, cysteine, either Tyr or Gly
GenProp02555-aminolevulinate biosynthesis
GenProp0261thiamin pyrophosphate (TPP) de novo biosynthesis
GenProp0274coenzyme B12 biosynthesis - overview
GenProp0300photosynthetic cofactor biosynthesis
GenProp0466molybdopterin biosynthesis
GenProp0752ergothioneine biosynthesis
GenProp0753mycothiol biosynthesis
GenProp0759para-aminobenzoic acid (PABA) biosynthesis from chorismate
GenProp07927,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) biosynthesis
GenProp0836menaquinone biosynthesis
GenProp0860tryptophan tryptophylquinone modification of methylamine dehydrogenase
GenProp0862pyridoxal phosphate biosynthesis, PdxA/PdxJ pathway
GenProp0863pyridoxal phosphate biosynthesis, Pdx1/Pdx2 pathway
GenProp0891coenzyme M biosynthesis
GenProp0917mycofactocin system
GenProp0927bacillithiol biosynthesis
GenProp1064ovothiol biosynthesis
GenProp1096coenzyme B biosynthesis