Genome Property Definition Page

Name7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) biosynthesis
Description7,8-didemethyl-8-hydroxy-5-deazariboflavin, or FO, is the chromophore of coenzyme F420. Several enzymatic steps convert FO to F420 (see GenProp0791). FO is also the chromophore of a cyanobacterial DNA photoreactivating enzyme. The two key enzymes of this pathway, encoded by CofG and CofH in Methanocaldococcus jannaschii, are encoded by fusion proteins in certain lineages. The fusion protein in Mycobacterium smegmatis is designated FbiC (F420 BIosynthesis C). The precursors are a pyrimidine intermediate from riboflavin biosynthesis and the 4-hydroxyphenylpyruvate precursor of tyrosine, so expressing FbiC in Escherichia coli is sufficient for FO biosynthesis.
JCVI RoleOther
Parent PropertyGenProp0184: cofactor biosynthesis
Literature References
[ 1 ] Eker AP, Kooiman P, Hessels JK, Yasui A   DNA photoreactivating enzyme from the cyanobacterium Anacystis nidulans.   J Biol Chem. 1990 May 15;265(14):8009-15.  PMID 2110564
[ 2 ] Choi KP, Kendrick N, Daniels L   Demonstration that fbiC is required by Mycobacterium bovis BCG for coenzyme F(420) and FO biosynthesis.   J Bacteriol. 2002 May;184(9):2420-8.  PMID 11948155
[ 3 ] Graham DE, Xu H, White RH   Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis.   Arch Microbiol. 2003 Dec;180(6):455-64. Epub 2003 Oct 31.  PMID 14593448
Gene Ontology TermGO:0009108: coenzyme biosynthetic process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
FO synthase, CofG subunitCofGYESTIGR03550 (HMM): 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunitGO:0009108: coenzyme biosynthetic process
FO synthase, CofH subunitCofHYESTIGR03551 (HMM): 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunitGO:0009108: coenzyme biosynthetic process

Parent Properties
GenProp0184cofactor biosynthesis

Sibling Properties
GenProp0001chorismate biosynthesis via shikimate
GenProp0030glutathione biosynthesis
GenProp0036biotin biosynthesis
GenProp0038tetrahydrofolate biosynthesis from GTP and PABA
GenProp0057NAD(P) biosynthesis from L-aspartate and DHAP
GenProp0112riboflavin, FAD and FMN from GTP and ribulose-5-P
GenProp0124pantothenate biosynthesis from aspartate and 2-oxoisovalerate
GenProp0136ubiquinone biosynthesis from chorismate, aerobic
GenProp0140iron-sulfur cluster assembly systems
GenProp0170coenzyme PQQ biosynthesis
GenProp0171coenzyme A biosynthesis from pantothenate
GenProp0194siroheme biosynthesis from uroporphrinogen-III
GenProp0220uroporhyrinogen III biosynthesis from 5-aminolevulinate
GenProp0221protoporphyrin IX biosynthesis from uroporphyrinogen III
GenProp0222protoheme from protoporphyrin IX
GenProp0250thiamine: hydroxyethylthiazole from 1-deoxy-D-xylulose-5-phosphate, cysteine, either Tyr or Gly
GenProp02555-aminolevulinate biosynthesis
GenProp0261thiamin pyrophosphate (TPP) de novo biosynthesis
GenProp0274coenzyme B12 biosynthesis - overview
GenProp0300photosynthetic cofactor biosynthesis
GenProp0466molybdopterin biosynthesis
GenProp0752ergothioneine biosynthesis
GenProp0753mycothiol biosynthesis
GenProp0759para-aminobenzoic acid (PABA) biosynthesis from chorismate
GenProp0791coenzyme F420 biosynthesis
GenProp0836menaquinone biosynthesis
GenProp0860tryptophan tryptophylquinone modification of methylamine dehydrogenase
GenProp0862pyridoxal phosphate biosynthesis, PdxA/PdxJ pathway
GenProp0863pyridoxal phosphate biosynthesis, Pdx1/Pdx2 pathway
GenProp0891coenzyme M biosynthesis
GenProp0917mycofactocin system
GenProp0927bacillithiol biosynthesis
GenProp1064ovothiol biosynthesis
GenProp1096coenzyme B biosynthesis