Genome Property Definition Page
| Accession | GenProp0326 | |
| Name | protein sorting system, PEP-CTERM/exosortase (generic) | |
| Type | SYSTEM | |
| Description | This Genome Property describes a proposed protein sorting system associated with exopolysaccharide loci in many Gram-negative bacteria. One hallmark is the presence of a paralogous domain at the C-termini of a substantial number of proteins in a single genome, usually from four to sixty-five. Features of this domain, described by TIGRFAMs model TIGR02595, are a conserved motif PEP, a transmembrane domain, and a cluster of basic residues for a total length of about 24 amino acids. We call this domain PEP-CTERM, and note that is shares a number of properties with the the LPXTG-type Gram-positive cell-wall targeting sequence. Another hallmark of this system is the presence of one or more homologs of EpsH (TIGR02602), a highly hydrophobic protein that spans the membrane eight or more times. Its surrounding genes nearly always pertain to exopolysaccharide biosynthesis. We have proposed that TIGR02602 models a transpeptidase that cleaves PEP-CTERM proteins, attaches them transiently to an active site Cys, then attaches them covalently to an unknown substrate for sorting to the outer surface of the cell, closely analogous to the action of sortase in Gram-positive bacteria. We suggest the name exosortase for EpsH homologs involved in this process. In Proteobacterial species with a the PEP-CTERM/exsortase system, a sensor histidine kinase (TIGR02916) and a sigma-54-dependent DNA-binding response regulator (TIGR02915) are present and appear to bind a conserved regulatory site found upstream of most PEP-CTERM genes. | |
| Parent Property | GenProp0069: cell surface component GenProp0179: protein transport | |
| Literature References |
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Components
| Step Name | Step Num | Required | Evidence (Method) | Evidence Go Terms |
|---|---|---|---|---|
| PEP-CTERM motif | CTERM | YES | TIGR02595 (HMM): PEP-CTERM protein-sorting domain PF07589 (HMM): PEP-CTERM motif | |
| EpsH family 8TM protein | EpsH | YES | TIGR02602 (HMM): exosortase | |
| EpsI protein/fusion | EpsI | NO | TIGR02914 (HMM): EpsI family protein |
Parent Properties
| Accession | Name |
|---|---|
| GenProp0069 | cell surface component |
| GenProp0179 | protein transport |
Sibling Properties
| Accession | Name |
|---|---|
| GenProp0007 | flagella |
| GenProp0012 | outer membrane |
| GenProp0022 | S-layer |
| GenProp0056 | type IV pilus |
| GenProp0062 | capsule |
| GenProp0158 | peptidoglycan(murein) biosynthesis |
| GenProp0297 | lipopolysaccharide biosynthesis |
| GenProp0626 | protein sorting system, sortase type, LPXTG/SrtA class |
| GenProp0664 | protein sorting system, sortase type, SrtB class |
| GenProp0725 | outer membrane protein assembly complex |
| GenProp0811 | S-layer homology domain-mediated cell wall binding |
| GenProp0847 | exosporium |
| GenProp0857 | anchored repeat-class ABC transporter, P. acnes type |
| GenProp0859 | protein sorting system, proteobacterial dedicated sortase type |
| GenProp0052 | type III secretion |
| GenProp0053 | type II secretion |
| GenProp0054 | type IV secretion |
| GenProp0059 | type I secretion |
| GenProp0127 | Tat (Sec-independent) protein export |
| GenProp0205 | signal recognition system, bacterial |
| GenProp0207 | lipoprotein localization system lolABCDE |
| GenProp0209 | Sec system preprotein translocase |
| GenProp0295 | prokaryotic N-terminal cleavage/methylation, type IV pilin-like |
| GenProp0669 | protein sorting system, putative, exosortase G class |
| GenProp0735 | type VI secretion |
| GenProp0778 | protein sorting system, GlyGly-CTERM/rhombosortase |
| GenProp0844 | accessory Sec system for serine-rich glycoprotein transport |
| GenProp0845 | protein sorting system, KxYKxGKxW class |
| GenProp0904 | type VII secretion, Actinobacteria form |
| GenProp0905 | type VII secretion, Firmicutes form |
| GenProp0928 | OMP chaperone system: SurA-Skp-DegP |
| GenProp0957 | protein sorting system, SipW class |
| GenProp0978 | protein sorting system, PGF-CTERM/archaeosortase A |
| GenProp0979 | protein sorting system, PEF-CTERM/archaeosortase C |
| GenProp0980 | protein sorting system, putative, exosortase F class |
| GenProp0983 | protein sorting system, VPXXXP-CTERM/archaeosortase B |
| GenProp0985 | protein sorting system, VPDSG-CTERM/exosortase C |
| GenProp0986 | protein sorting system, VPLPA-CTERM/exosortase D |
| GenProp0987 | protein sorting system, cyano-PEP-CTERM class |
| GenProp0989 | protein sorting system, VPEID-CTERM/exosortase E |
| GenProp0993 | protein sorting system, PIP-CTERM/archaeosortase D |
| GenProp0996 | protein sorting system, IPTLxxWG-CTERM/exosortase H |
| GenProp0999 | Por secretion system |
| GenProp1005 | protein sorting system, putative, exosortase J class |
| GenProp1054 | protein sorting system, MSEP-CTERM/exosortase K |
| GenProp1055 | protein sorting system, PEFG-CTERM/thaumarchaeosortase |
| GenProp1074 | type V secretion |
| GenProp1087 | protein sorting system, vault protein/exosortase N |
| GenProp1102 | protein sorting system, CGP-CTERM/unknown of Thermococcaceae |
| GenProp1103 | protein sorting system, Synergist-CTERM/unknown of Synergistetes |