Genome Property Definition Page

Nameisoleucine biosynthesis from threonine and pyruvate
DescriptionThe branched amino acid isoleucine is biosynthesized from the central intermediary metabolite pyruvate and the amino acid threonine via a five step pathway. Four enzymes in this pathway are common to the pathways for the biosynthesis of leucine and valine, although specialized versions of these enzymes may exist in certain species which have a narrowed substrate range or amino acid-specific regulatory mechanisms. Unlike those other two pathways in which two molecules of pyruvate are condensed, here threonine is converted first to 2-oxobutyrate and then condensed with pyruvate. Thus, the enzymes common to the pathways are acting on distinct substrates. 2-oxobutyrate for this pathway may also be generated by a separate path from glutamate via mesaconate in certain organisms. The penultimate intermediate in this pathway, 2-oxo-3-methylvalerate may also be incorporated into branched-chain fatty acids
JCVI RoleAspartate family
Parent PropertyGenProp0126: amino acid biosynthesis
Web ReferencesIUBMB: Isoleucine and Valine Biosynthesis
KEGG: Valine, leucine and isoleucine biosynthesis
Gene Ontology TermGO:0009097: isoleucine biosynthetic process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
threonine dehydrataseIle_1YESTIGR01127 (HMM): threonine ammonia-lyase
TIGR01124 (HMM): threonine ammonia-lyase, biosynthetic
TIGR02079 (HMM): threonine dehydratase
GO:0009097: isoleucine biosynthetic process
GO:0009097: isoleucine biosynthetic process
GO:0009097: isoleucine biosynthetic process
acetohydroxyacid synthase, large subunitIle_2aYESTIGR00118 (HMM): acetolactate synthase, large subunit, biosynthetic typeGO:0009097: isoleucine biosynthetic process
acetohydroxyacid synthase, small subunitIle_2bNOTIGR00119 (HMM): acetolactate synthase, small subunitGO:0009097: isoleucine biosynthetic process
ketol-acid reductoisomeraseIle_3YESTIGR00465 (HMM): ketol-acid reductoisomeraseGO:0009097: isoleucine biosynthetic process
dihydroxy-acid dehydrataseIle_4YESTIGR00110 (HMM): dihydroxy-acid dehydrataseGO:0009097: isoleucine biosynthetic process
branched-chain amino acid aminotransferaseIle_5NOTIGR01122 (HMM): branched-chain amino acid aminotransferase
TIGR01123 (HMM): branched-chain amino acid aminotransferase
GO:0009097: isoleucine biosynthetic process
GO:0009097: isoleucine biosynthetic process

Parent Properties
GenProp0126amino acid biosynthesis

Sibling Properties
GenProp0001chorismate biosynthesis via shikimate
GenProp0016selenocysteine system
GenProp0037tryptophan biosynthesis from ribose-5-phosphate
GenProp0039phenylalanine biosynthesis from chorismate
GenProp0045tyrosine biosynthesis from chorismate
GenProp0087arginine biosynthesis from glutatmate via N-acetylglutamate
GenProp0109histidine biosynthesis from ribose-5-phosphate
GenProp0111proline biosynthesis from glutamate
GenProp0117arginine biosynthesis from ornithine, carbamoyl-p and aspartate
GenProp0118ornithine biosynthesis from glutamate, acetylated branch
GenProp0159threonine biosynthesis from aspartate semialdehyde
GenProp0160aspartate semialdehyde biosynthesis from aspartate
GenProp0161methionine biosynthesis from homoserine
GenProp0163valine biosynthesis from pyruvate
GenProp0164leucine biosynthesis from pyruvate and acetyl-CoA
GenProp0178glycine cleavage system
GenProp0199lysine biosynthesis
GenProp0304cysteine biosynthesis, tRNA-dependent
GenProp0305cysteine biosynthesis
GenProp0729methionine salvage from methylthioadenosine
GenProp0797selenocysteinyl-tRNA, PSTK/SepSecS pathway