Genome Property Definition Page
| Accession | GenProp0162 |
| Name | isoleucine biosynthesis from threonine and pyruvate |
| Type | PATHWAY |
| Description | The branched amino acid isoleucine is biosynthesized from the central intermediary metabolite pyruvate and the amino acid threonine via a five step pathway. Four enzymes in this pathway are common to the pathways for the biosynthesis of leucine and valine, although specialized versions of these enzymes may exist in certain species which have a narrowed substrate range or amino acid-specific regulatory mechanisms. Unlike those other two pathways in which two molecules of pyruvate are condensed, here threonine is converted first to 2-oxobutyrate and then condensed with pyruvate. Thus, the enzymes common to the pathways are acting on distinct substrates. 2-oxobutyrate for this pathway may also be generated by a separate path from glutamate via mesaconate in certain organisms. The penultimate intermediate in this pathway, 2-oxo-3-methylvalerate may also be incorporated into branched-chain fatty acids |
| JCVI Role | Aspartate family |
| Parent Property | GenProp0126: amino acid biosynthesis |
| Web References | IUBMB: Isoleucine and Valine Biosynthesis KEGG: Valine, leucine and isoleucine biosynthesis |
| Gene Ontology Term | GO:0009097: isoleucine biosynthetic process (biological_process) |
Components
| Step Name | Step Num | Required | Evidence (Method) | Evidence Go Terms |
|---|---|---|---|---|
| threonine dehydratase | Ile_1 | YES | TIGR01127 (HMM): threonine ammonia-lyase TIGR01124 (HMM): threonine ammonia-lyase, biosynthetic TIGR02079 (HMM): threonine dehydratase | GO:0009097: isoleucine biosynthetic process GO:0009097: isoleucine biosynthetic process GO:0009097: isoleucine biosynthetic process |
| acetohydroxyacid synthase, large subunit | Ile_2a | YES | TIGR00118 (HMM): acetolactate synthase, large subunit, biosynthetic type | GO:0009097: isoleucine biosynthetic process |
| acetohydroxyacid synthase, small subunit | Ile_2b | NO | TIGR00119 (HMM): acetolactate synthase, small subunit | GO:0009097: isoleucine biosynthetic process |
| ketol-acid reductoisomerase | Ile_3 | YES | TIGR00465 (HMM): ketol-acid reductoisomerase | GO:0009097: isoleucine biosynthetic process |
| dihydroxy-acid dehydratase | Ile_4 | YES | TIGR00110 (HMM): dihydroxy-acid dehydratase | GO:0009097: isoleucine biosynthetic process |
| branched-chain amino acid aminotransferase | Ile_5 | NO | TIGR01122 (HMM): branched-chain amino acid aminotransferase TIGR01123 (HMM): branched-chain amino acid aminotransferase | GO:0009097: isoleucine biosynthetic process GO:0009097: isoleucine biosynthetic process |
Parent Properties
| Accession | Name |
|---|---|
| GenProp0126 | amino acid biosynthesis |
Sibling Properties
| Accession | Name |
|---|---|
| GenProp0001 | chorismate biosynthesis via shikimate |
| GenProp0016 | selenocysteine system |
| GenProp0037 | tryptophan biosynthesis from ribose-5-phosphate |
| GenProp0039 | phenylalanine biosynthesis from chorismate |
| GenProp0045 | tyrosine biosynthesis from chorismate |
| GenProp0087 | arginine biosynthesis from glutatmate via N-acetylglutamate |
| GenProp0109 | histidine biosynthesis from ribose-5-phosphate |
| GenProp0111 | proline biosynthesis from glutamate |
| GenProp0117 | arginine biosynthesis from ornithine, carbamoyl-p and aspartate |
| GenProp0118 | ornithine biosynthesis from glutamate, acetylated branch |
| GenProp0159 | threonine biosynthesis from aspartate semialdehyde |
| GenProp0160 | aspartate semialdehyde biosynthesis from aspartate |
| GenProp0161 | methionine biosynthesis from homoserine |
| GenProp0163 | valine biosynthesis from pyruvate |
| GenProp0164 | leucine biosynthesis from pyruvate and acetyl-CoA |
| GenProp0178 | glycine cleavage system |
| GenProp0199 | lysine biosynthesis |
| GenProp0304 | cysteine biosynthesis, tRNA-dependent |
| GenProp0305 | cysteine biosynthesis |
| GenProp0729 | methionine salvage from methylthioadenosine |
| GenProp0797 | selenocysteinyl-tRNA, PSTK/SepSecS pathway |