Genome Property Definition Page

NameGatABC aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase complex
DescriptionThe GatABC heterotrimer acts as an alternative to direct aminoacylation of tRNA(Gln), tRNA(Asn), or both with its respective amino acid. Rather, the tRNA may be misacylated with Glu in the place of Gln, or Asp in the place of Asn. The phylogenetic ranges over which GatABC orthologs act on Glu-tRNA(Gln), Asp-tRNA(Asn), or both are not fully worked out. It is both in Chlamydia, the Thermus-Deinococcus lineage, and Acidithiobacillus ferrooxidans. In the Archaea, GatDE act on Glu-tRNA(Gln) while GatABC act on Asp-tRNA(Asn). In Lactobacillus and possibly all Firmicutes, GatABC acts on Glu-tRNA(Gln) only. Note that the amidotransferase reaction is a mechanism not only to charge a tRNA with Asn or Gln, but to complete the synthesis of that amino acid.
JCVI RoletRNA aminoacylation
Parent PropertyGenProp0197: protein biosynthesis
GenProp0258: tRNA aminoacylation
Web ReferencesMetaCyc Pathway: Glu-tRNA(Gln) amidotransferase

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
glutamyl-tRNA(Gln) amidotransferase, A subunitgatAYESTIGR00132 (HMM): aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
glutamyl-tRNA(Gln) amidotransferase, B subunitgatBYESTIGR00133 (HMM): aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit
PF01162 (HMM):
glutamyl-tRNA(Gln) amidotransferase, C subunitgatCYESTIGR00135 (HMM): aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, C subunit
PF02686 (HMM): glutamyl-tRNA(Gln) amidotransferase, C subunit

Parent Properties
GenProp0197protein biosynthesis
GenProp0258tRNA aminoacylation

Sibling Properties
GenProp0016selenocysteine system
GenProp0132transcription termination - antitermination Nus factors
GenProp0148release factor 2 programmed frameshift
GenProp0174ribosome, bacterial, large subunit
GenProp0175ribosome, bacterial, small subunit
GenProp0189tRNA-Gln direct aminoacylation
GenProp0211tmRNA trans-translation system
GenProp0258tRNA aminoacylation
GenProp0259tRNA-Asn direct aminoacylation
GenProp0304cysteine biosynthesis, tRNA-dependent
GenProp0634Sigma-54 (RpoN) systems
GenProp0692selenouridine-containing tRNA
GenProp0704tRNA U34 carboxymethylaminomethyl modification
GenProp0740translation initiation, bacterial
GenProp0741translation elongation, bacterial
GenProp0746translation termination, bacterial
GenProp0812rho-dependent termination