Genome Property Definition Page

Namehydrogenase, mono-iron type
DescriptionThis property describes 5,10-methenyltetrahydromethanopterin hydrogenase (HmdA), also called mono-iron hydrogenase or iron-sulfur cluster-free hydrogenase, together with a few of its accessory proteins: HmdB (a radical SAM protein) and HmdC. The enzyme contains a novel cofactor with an Fe ion ligated by two CO molecules, a cysteine side chain, a guanylyl pyridinol cofactor, and an uncharacterized additional ligand.
JCVI RoleMethanogenesis
Parent PropertyGenProp0070: energy metabolism
Literature References
[ 1 ]McGlynn SE, Boyd ES, Shepard EM, Lange RK, Gerlach R, Broderick JB, Peters JW  Identification and characterization of a novel member of the radical AdoMet enzyme superfamily and implications for the biosynthesis of the Hmd hydrogenase active site cofactor.  J Bacteriol. 2010 Jan;192(2):595-8. Epub 2009 Nov 6.  PMID 19897660
[ 2 ]Guo Y, Wang H, Xiao Y, Vogt S, Thauer RK, Shima S, Volkers PI, Rauchfuss TB, Pelmenschikov V, Case DA, Alp EE, Sturhahn W, Yoda Y, Cramer SP  Characterization of the Fe site in iron-sulfur cluster-free hydrogenase (Hmd) and of a model compound via nuclear resonance vibrational spectroscopy (NRVS).  Inorg Chem. 2008 May 19;47(10):3969-77. Epub 2008 Apr 12.  PMID 18407624

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
5,10-methenyltetrahydromethanopterin hydrogenasehmdAYESTIGR01723 (HMM): 5,10-methenyltetrahydromethanopterin hydrogenase
HmdA cofactor biosynthesis rSAM protein HmdBhmdBYESTIGR03957 (HMM): 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB
HmdA cofactor biosynthesis protein HmdChmdCYESTIGR03958 (HMM): 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdC

Parent Properties
GenProp0070energy metabolism

Sibling Properties
GenProp0023glyoxylate shunt
GenProp0033TCA cycle
GenProp0120pentose phosphate cycle
GenProp0131electron transport
GenProp0243ATP synthases
GenProp0459D-xylose utilization (conversion to D-xylulose-5-phosphate)
GenProp0468Entner-Doudoroff pathway
GenProp0473arsenite oxidase
GenProp0611aerobic respiration
GenProp0636respiratory nitrate reductase
GenProp0637anaerobic dimethyl sulfoxide reductase
GenProp0638trimethylamine N-oxide reductase system
GenProp0682formate-dependent nitrite reductase, seven-gene type
GenProp0683cytochrome c nitrite reductase NrfHA
GenProp0695cytochrome b558/566
GenProp0723hydrogenase, nickel and F420-dependent
GenProp0737anaerobic glycerol-3-phosphate dehydrogenase complex
GenProp0755pyruvate conversion to propionate, anaerobic, FAD/NAD-regenerating
GenProp0756fumarate reductase complex
GenProp0764DMSO reductase family type II enzyme
GenProp08412-oxoacid:ferredoxin oxidoreductase
GenProp0856bacterio-opsin system
GenProp0914hydrogenase, [FeFe]-dependent
GenProp1089B12-dependent trimeric diol/glycerol dehydratase
GenProp1101quinoprotein cytochrome relay system