Genome Property Definition Page

NameRNA repair, Hen1/Pnkp system
DescriptionThis property describe the combination of an RNA modification enzyme, the methyltransferase Hen1, and an RNA repair enzyme, polynucleotide kinase-phosphatase, as found in model species Clostridium thermocellum and in many other bacteria in a two-gene cassette. This pairing may allow RNA cleaved by ribotoxins to be repaired, and to be modified at the site of repair to prevent the same breakage from occurring again.
JCVI RoleRNA processing
Parent PropertyGenProp0819: RNA metabolism
Literature References
[ 1 ]Keppetipola N, Shuman S  Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage lambda phosphatase.  Nucleic Acids Res. 2007;35(22):7721-32. Epub 2007 Nov 5.  PMID 17986465
[ 2 ]Jain R, Shuman S  Bacterial Hen1 is a 3' terminal RNA ribose 2'-O-methyltransferase component of a bacterial RNA repair cassette.  RNA. 2010 Feb;16(2):316-23. Epub 2009 Dec 9.  PMID 20007328
Gene Ontology TermGO:0042245: RNA repair (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
3' terminal RNA ribose 2'-O-methyltransferase Hen1Hen1YESTIGR04074 (HMM): 3' terminal RNA ribose 2'-O-methyltransferase Hen1GO:0042245: RNA repair
polynucleotide kinase-phosphatasePnkpYESTIGR04075 (HMM): polynucleotide kinase-phosphataseGO:0042245: RNA repair

Parent Properties
GenProp0819RNA metabolism

Sibling Properties
GenProp0258tRNA aminoacylation
GenProp0262RNA polymerase, bacterial
GenProp04826S RNA (eubacterial RNA polymerase regulator)
GenProp0677queuosine (Q-tRNA) biosynthesis from preQ0
GenProp0692selenouridine-containing tRNA
GenProp0704tRNA U34 carboxymethylaminomethyl modification
GenProp0898RNA ligase/unknown protein pair
GenProp1060tRNA N6-threonylcarbamoyladenosine modification
GenProp1077tRNA(Pro) cmo5UGG modification
GenProp108216S rRNA C1402 m(4)Cm modification