Genome Property Definition Page

Namenitrile hydratase
DescriptionThe enzyme nitrile hydratase (EC catalyzes the interconversion of an aliphatic amide to a nitrile plus H20. The enzyme is closely related to thiocyanate hydrolase (EC The nitrile hydratase alpha subunit contains active site Cys residues that undergo covalent modification, after which it binds either Fe(III) or Co(III). A CXCC-containing protein related to the cobalamin biosynthesis protein CobW is described as a nitrile hydratase activator and is often found in the nitrile hydratase operon. However, the protein family described by TIGR03889, also commonly annotated as a nitrile hydratase accessory protein, is found in a larger fraction of nitrile hydratase operons.
Parent PropertyGenProp0202: nitrogen metabolism
Literature References
[ 1 ]Harrop TC, Mascharak PK  Fe(III) and Co(III) centers with carboxamido nitrogen and modified sulfur coordination: lessons learned from nitrile hydratase.  Acc Chem Res. 2004 Apr;37(4):253-60.  PMID 15096062

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
nitrile hydratase activator, CobW familynitCXCCNOPF02492 (HMM-CLUST): CobW/P47K family protein
nitrile hydratase accessory proteinnit_accNOTIGR03889 (HMM): nitrile hydratase accessory protein
nitrile hydratase, alpha subunitnit_alpYESTIGR01323 (HMM): nitrile hydratase, alpha subunit
nitrile hydratase, beta subunitnit_betYESTIGR03888 (HMM): nitrile hydratase, beta subunit

Parent Properties
GenProp0202nitrogen metabolism

Sibling Properties
GenProp0029nitrogen fixation
GenProp0200periplasmic nitrate reductase
GenProp0666membrane-associated monooxygenase (methane/ammonia)
GenProp0682formate-dependent nitrite reductase, seven-gene type
GenProp0683cytochrome c nitrite reductase NrfHA
GenProp0814urea utilization