Genome Property Definition Page

Namepyruvate formate-lyase system
DescriptionThis property consists of the glycine radical enzyme, pyruvate-formate lyase (EC, also called formate C-acetyltransferase) and its activating enzyme, [Formate-C-acetyltransferase]-activating enzyme (EC In most Proteobacteria, the gene pair is accompanied by the formate channel gene focA. FocA forms a pentameric channel that can pass formate but not water, and that belongs to the same transporter family as nitrate transporter NirC.
Parent PropertyGenProp0075: protein modification, prosthetic groups and cofactors
Literature References
[ 1 ]Wagner AF, Schultz S, Bomke J, Pils T, Lehmann WD, Knappe J  YfiD of Escherichia coli and Y06I of bacteriophage T4 as autonomous glycyl radical cofactors reconstituting the catalytic center of oxygen-fragmented pyruvate formate-lyase.  Biochem Biophys Res Commun. 2001 Jul 13;285(2):456-62.  PMID 11444864

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
pyruvate formate-lyasePFLYESTIGR01774 (HMM): glycyl radical enzyme, PFL2/glycerol dehydratase family
TIGR01255 (HMM): formate acetyltransferase
pyruvate formate-lyase activating enzymePFLAYESTIGR02493 (HMM): pyruvate formate-lyase 1-activating enzyme
formate transporterPF_FocANOPF01226 (HMM-CLUST): formate/nitrite transporter
autonomous glycyl radical cofactorspareNOTIGR04365 (HMM): autonomous glycyl radical cofactor GrcA

Parent Properties
GenProp0075protein modification, prosthetic groups and cofactors

Sibling Properties
GenProp0002coenzyme F420 utilization
GenProp0016selenocysteine system
GenProp0061lipoprotein system lgt/lsp/lnt
GenProp0127Tat (Sec-independent) protein export
GenProp0284cobalamin-utilizing enzymes
GenProp0295prokaryotic N-terminal cleavage/methylation, type IV pilin-like
GenProp0726selenium-dependent molybdenum hydroxylase system
GenProp0745lipoyl-protein attachment
GenProp0860tryptophan tryptophylquinone modification of methylamine dehydrogenase
GenProp0918anaerobic sulfatase/maturase system
GenProp0923mycothiol system
GenProp0933bacillithiol utilization
GenProp0938glycine radical enzyme system, YjjI/YjjW pair