Genome Property Definition Page

Nameproteasome-targeting modification by pupylation
DescriptionPupylation is the process by which the small protein Pup is conjugated to proteins through its C-terminus to a lysine epsilon-amino group of the target. This modification, analogous to ubiquitination in eukaryotes, targets proteins for degradation by archaeal-type proteasomes in Mycobacterium tuberculosis and related Actinobacteria.
JCVI RoleDegradation of proteins, peptides, and glycopeptides
Parent PropertyGenProp0301: protein degradation
Literature References
[ 1 ] Pearce MJ, Mintseris J, Ferreyra J, Gygi SP, Darwin KH  Ubiquitin-Like Protein Involved in the Proteasome Pathway of Mycobacterium tuberculosis.   Science. 2008 Oct 2.  PMID 18832610
[ 2 ] Festa RA, Pearce MJ, Darwin KH   Characterization of the proteasome accessory factor (paf) operon in Mycobacterium tuberculosis.   J Bacteriol. 2007 Apr;189(8):3044-50.  PMID 17277063
Gene Ontology TermGO:0019941: modification-dependent protein catabolic process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
20S proteasomePROSOMEYESGenProp0834 (GENPROP): proteasome, bacterial
GO:0019941: modification-dependent protein catabolic process
ubiquitin-like protein PuppupYESTIGR03687 (HMM): ubiquitin-like protein PupGO:0019941: modification-dependent protein catabolic process
AAA family ATPase, pupylation-associatedpup_AAAYESTIGR03689 (HMM): proteasome ATPaseGO:0019941: modification-dependent protein catabolic process
Pup ligasepup_ligYESTIGR03686 (HMM): Pup--protein ligase
TIGR03688 (HMM): proteasome accessory factor PafA2
GO:0019941: modification-dependent protein catabolic process
GO:0019941: modification-dependent protein catabolic process

Parent Properties
GenProp0301protein degradation

Sibling Properties
GenProp0211tmRNA trans-translation system
GenProp0251clpXP degradation machine
GenProp0684FtsH/HflKC protease complex
GenProp0834proteasome, bacterial
GenProp0835ATP-dependent protease HslVU