Genome Property Definition Page

Accession

GenProp0794

Name

proposed N-acetyl sugar amidation module WbuXYZ

Type

SYSTEM

Description

The WbuX protein from E. coli and its homologs in P. aeruginosa (IfnA) and C. jejuni (PseA) have been proposed to function as N-acetyl sugar amidotransferases, forming the positively charged acetamidino moitey on both O-antigens and glycosylated flagellins [1,2]. These WbuX homologs are invariably associated with homologs of the HisH and HisF proteins (WbuYZ) which are proposed to act as a glutaminase and ammonium channel to provide sufficient ammonium ion under limiting conditions [3]. Occasionally the WbuX protein is found in multiple (tandem) copies which may indicate multiple substrates or multiple N-acetyl groups being modified on the same substrate.

Parent Property

GenProp0795: carbohydrate biosynthesis

Literature References

[ 1 ] King JD, Mulrooney EF, Vinogradov E, Kneidinger B, Mead K, Lam JS   lfnA from Pseudomonas aeruginosa O12 and wbuX from Escherichia coli O145 encode membrane-associated proteins and are required for expression of 2,6-dideoxy-2-acetamidino-L-galactose in lipopolysaccharide O antigen.   J Bacteriol. 2008 Mar;190(5):1671-9. Epub 2007 Dec 21.  PMID 18156256
[ 2 ] McNally DJ, Hui JP, Aubry AJ, Mui KK, Guerry P, Brisson JR, Logan SM, Soo EC   Functional characterization of the flagellar glycosylation locus in Campylobacter jejuni 81-176 using a focused metabolomics approach.   J Biol Chem. 2006 Jul 7;281(27):18489-98. Epub 2006 May 9.  PMID 16684771
[ 3 ] Feng L, Senchenkova SN, Tao J, Shashkov AS, Liu B, Shevelev SD, Reeves PR, Xu J, Knirel YA, Wang L   Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay.   J Bacteriol. 2005 Jan;187(2):758-64.  PMID 15629947

Components

Step Name	Step Num	Required	Evidence (Method)
N-acetyl sugar amidotransferase	WbuX	YES	TIGR03573 (HMM): N-acetyl sugar amidotransferase
HisH-like glutaminase WbuY	WbuY	YES	TIGR01855 (HMM-CLUST): imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
HisF-like proposed ammonium channel protein WbuZ	WbuZ	YES	TIGR03572 (HMM): glycosyl amidation-associated protein WbuZ 2918 (RULE_BASE)

Parent Properties

Accession	Name
GenProp0795	carbohydrate biosynthesis

Sibling Properties

Accession	Name
GenProp0183	dTDP-L-rhamnose biosynthesis from dTDP-4-dehydro-L-rhamnose
GenProp0264	glucosylglycerol biosynthesis
GenProp0297	lipopolysaccharide biosynthesis
GenProp0750	UDP-N-acetylglucosamine biosynthesis from fructose-6-phosphate
GenProp0766	anhydro-N-acetylmuramic acid to N-acetylglucosamine-phosphate
GenProp0793	CMP-N-acetylneuraminate biosynthesis from UDP-N-acetylglucosamine
GenProp0796	CMP-pseudaminic acid biosynthesis from UDP-N-acetylglucosamine
GenProp0811	S-layer homology domain-mediated cell wall binding