Genome Property Definition Page

NameTol-Pal system
DescriptionA complex of TolQ, TolR, and TolA in the inner membrane, interacting through TolA with a complex of TolB and Pal (also called OprL) present at the outer membrane, is pivotal to maintaining morphology and outer membrane integrity in many Gram-negative bacteria. The tol operon(s) routinely also contain ybgC (orf1) and ybgF (orf2) genes, although these are not clearly associated with mutant phenotypes. The TolA protein, homologous to TonB from a similar complex, is the most divergent, as it consists largely of very low complexity sequence. TolB contains a variable number of beta-propellar repeats.
Parent PropertyGenProp0012: outer membrane
GenProp0180: small molecule transport
Literature References
[ 1 ]Sturgis JN  Organisation and evolution of the tol-pal gene cluster.  J Mol Microbiol Biotechnol. 2001 Jan;3(1):113-22.  PMID 11200223
[ 2 ]Dubuisson JF, Vianney A, Hugouvieux-Cotte-Pattat N, Lazzaroni JC  Tol-Pal proteins are critical cell envelope components of Erwinia chrysanthemi affecting cell morphology and virulence.  Microbiology. 2005 Oct;151(Pt 10):3337-47.  PMID 16207916
[ 3 ]Llamas MA, Ramos JL, Rodriguez-Herva JJ  Transcriptional organization of the Pseudomonas putida tol-oprL genes.  J Bacteriol. 2003 Jan;185(1):184-95.  PMID 12486055
[ 4 ]Zhuang Z, Song F, Martin BM, Dunaway-Mariano D  The YbgC protein encoded by the ybgC gene of the tol-pal gene cluster of Haemophilus influenzae catalyzes acyl-coenzyme A thioester hydrolysis.  FEBS Lett. 2002 Apr 10;516(1-3):161-3.  PMID 11959124
[ 5 ]Cascales E, Lloubes R, Sturgis JN  The TolQ-TolR proteins energize TolA and share homologies with the flagellar motor proteins MotA-MotB.  Mol Microbiol. 2001 Nov;42(3):795-807.  PMID 11722743
[ 6 ]Cascales E, Lloubes R  Deletion analyses of the peptidoglycan-associated lipoprotein Pal reveals three independent binding sequences including a TolA box.  Mol Microbiol. 2004 Feb;51(3):873-85.  PMID 14731286
[ 7 ]Llamas MA, Rodriguez-Herva JJ, Hancock RE, Bitter W, Tommassen J, Ramos JL  Role of Pseudomonas putida tol-oprL gene products in uptake of solutes through the cytoplasmic membrane.  J Bacteriol. 2003 Aug;185(16):4707-16.  PMID 12896989

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
tol-pal system protein Pal (OprL)PalYESTIGR02802 (HMM): peptidoglycan-associated lipoprotein
TolATolAYESTIGR02794 (HMM): protein TolA
TolBTolBYESTIGR02800 (HMM): Tol-Pal system beta propeller repeat protein TolB
TolQTolQYESTIGR02796 (HMM): protein TolQ
TolRTolRYESTIGR02801 (HMM): protein TolR
tol-pal region thioesterase YbgCYbgCNOTIGR02799 (HMM): tol-pal system-associated acyl-CoA thioesterase
tol-pal region protein YbgFYbgFNOTIGR02795 (HMM): tol-pal system protein YbgF

Parent Properties
GenProp0012outer membrane
GenProp0180small molecule transport

Sibling Properties
GenProp0053type II secretion
GenProp0119PTS transport system
GenProp0129Na+-translocating NADH-quinone reductase
GenProp0135NADH dehydrogenase I
GenProp0172Potassium-transporting ATPase KdpFABC
GenProp0176TRAP-T (tripartite ATP-independent periplasmic transporters) family transporters
GenProp0190phosphate ABC transporter (pstSCAB-phoU)
GenProp0191sulfate/thiosulfate ABC transporter
GenProp0192molybdate ABC transporter
GenProp0236phosphonates ABC transport
GenProp0252thiamine/TPP ABC transporter ThiBPQ
GenProp0279cobalt import systems
GenProp0494nickel import ABC transporter
GenProp0543TonB-dependent transport
GenProp0651ectoine ABC transporter EhuABCD
GenProp0693fructose utilization as fructose-1,6-bisphosphate
GenProp0694glucose utilization as fructose-1,6-bisphosphate
GenProp07122-aminoethylphosphonate (AEP) ABC transporter, type I, PhnSTUV
GenProp07212-aminoethylphosphonate (AEP) ABC transporter, type II
GenProp0743urea ABC transporter UrtABCDE
GenProp0748choline ABC transporter, ChoXWV family
GenProp0749choline sulfate utilization
GenProp0828heme uptake system, NEAT-domain mediated
GenProp0853lantibiotic system, gallidermin/epidermin family
GenProp0873ion-motive antiporter cycles