Genome Property Definition Page

Nameurea carboxylase/allophanate hydrolase pathway
DescriptionThis two-step, ATP-dependent pathway for urea hydrolysis is an alternative to urease. Urease requires a transporter and accessory proteins to produce its nickel metallocenter, while this two-step system uses biotin. Urea carboxylase acts first in this pathway, requiring ATP and bicarbonate as substrates. Allophanate hydrolase acts next, but note that this enzyme may be found in systems that do not contain a urea carboxylase. In yeasts such as Saccaromyces cerevisiae, the two activities of this pathway are encoded in a fusion protein, designated urea amidolyase. This pathway appears to function primarily for the purpose of obtaining nitrogen in the form of ammonia. A pair of uncharacterized, tandem, homologous genes, restricted to organisms with this system and adjacent to the known genes, has been added to this Genome Property.
JCVI RoleNitrogen metabolism
Parent PropertyGenProp0814: urea utilization
Literature References
[ 1 ]Kanamori T, Kanou N, Atomi H, Imanaka T  Enzymatic characterization of a prokaryotic urea carboxylase.  J Bacteriol 2004 May;186(9):2532-9.  PMID 15090492
Gene Ontology TermGO:0043419: urea catabolic process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
ABC transporter periplasmic binding protein, urea carboxylase regionABC_binNOTIGR03427 (HMM): ABC transporter periplasmic binding protein, urea carboxylase regionGO:0043419: urea catabolic process
allophanate hydrolaseallophYESTIGR02713 (HMM): allophanate hydrolaseGO:0043419: urea catabolic process
urea carboxylaseucaYESTIGR02712 (HMM): urea carboxylaseGO:0043419: urea catabolic process
urea carboxylase-associated protein 1unchar1YESTIGR03424 (HMM): urea carboxylase-associated protein 1GO:0043419: urea catabolic process
urea carboxylase-associated protein 2unchar2YESTIGR03425 (HMM): urea carboxylase-associated protein 2GO:0043419: urea catabolic process

Parent Properties
GenProp0814urea utilization

Sibling Properties
GenProp0743urea ABC transporter UrtABCDE