Genome Property Definition Page

Accession

GenProp0292

Name

ethanolamine degradation organelle

Type

SYSTEM

Description

Ethanolamine can be broken down to acetaldehyde by the action of ethanolamine ammonia lyase, an adenosyl cobalamin (vitamin B12) dependent enzyme. In its simplest form this system consists only of the two subunits of the enzyme, but ususally also includes a transporter for ethanolamine and may include a coexpressed acetaldehyde dehydrogenase to ensure that the toxic aldehyde is efficiently converted to acetyl-CoA. This property encompasses the more complex version of this system which includes additional genes coding for the proteinaceous coat of a specialized organelle as well as other genes for the efficienct maintenance and regulation of the lyase, handling of the product and import of the substrate. Presumably, this organellar system in organisms which may rely on ethanolamine as a primary carbon source.

Parent Property

GenProp0294: ethanolamine degradation proteinaceous organelle

Literature References

[ 1 ]Faust LP, Babior BM  Overexpression, purification, and some properties of the AdoCbl-dependent ethanolamine ammonia-lyase from Salmonella typhimurium.  Arch Biochem Biophys 1992 Apr;294(1):50-4.  PMID 1550360
[ 2 ]Mori K, Bando R, Hieda N, Toraya T  Identification of a reactivating factor for adenosylcobalamin-dependent ethanolamine ammonia lyase.  J Bacteriol 2004 Oct;186(20):6845-54.  PMID 15466038
[ 3 ]Sheppard DE, Penrod JT, Bobik T, Kofoid E, Roth JR  Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica.  J Bacteriol 2004 Nov;186(22):7635-44.  PMID 15516577
[ 4 ]Penrod JT, Mace CC, Roth JR  A pH-sensitive function and phenotype: evidence that EutH facilitates diffusion of uncharged ethanolamine in Salmonella enterica.  J Bacteriol 2004 Oct;186(20):6885-90.  PMID 15466042
[ 5 ]Huseby DL, Roth JR  Evidence that a metabolic microcompartment contains and recycles private cofactor pools.  J Bacteriol. 2013 Jun;195(12):2864-79. doi: 10.1128/JB.02179-12.  PMID 23585538

Web References

Glycerophospholipid metabolism

Gene Ontology Term

GO:0046336: ethanolamine catabolic process (biological_process)

Components

Step Name	Step Num	Required	Evidence (Method)	Evidence Go Terms
phosphotransacetylase	PtrnsAc	NO	PF01515 (HMM-CLUST): phosphate acetyl/butyryl transferase	GO:0046336: ethanolamine catabolic process
conserved hypothetical in ethanolamine operon	eut-chp	NO	TIGR02536 (HMM): ethanolamine utilization protein	GO:0046336: ethanolamine catabolic process
reactivating factor for ethanolamine ammonia lyase	eutA	YES	PF06277 (HMM): ethanolamine utilization protein EutA	GO:0046336: ethanolamine catabolic process
ethanolamine ammonia lyase, large subunit	eutB	YES	PF06751 (HMM): ethanolamine ammonia-lyase, large subunit	GO:0046336: ethanolamine catabolic process
ethanolamine ammonia lyase, small subunit	eutC	YES	PF05985 (HMM): ethanolamine ammonia-lyase, light subunit	GO:0046336: ethanolamine catabolic process
acetaldehyde dehydrogenase (acetylating)	eutE	YES	TIGR02518 (HMM-CLUST): acetaldehyde dehydrogenase (acetylating) PF00171 (HMM-CLUST): aldehyde dehydrogenase (NAD) family protein PF00171 (NOCLUST): aldehyde dehydrogenase (NAD) family protein	GO:0046336: ethanolamine catabolic process GO:0046336: ethanolamine catabolic process GO:0046336: ethanolamine catabolic process
alcohol dehydrogenase, ethanolamine operon related	eutG	YES	PF00465 (HMM-CLUST): alcohol dehydrogenase, iron-containing	GO:0046336: ethanolamine catabolic process
ethanolamine permease	eutH	YES	TIGR00908 (HMM): ethanolamine permease PF04346 (HMM): ethanolamine utilisation protein EutH	GO:0046336: ethanolamine catabolic process GO:0046336: ethanolamine catabolic process
ethanolamine utilization protein EutJ	eutJ	NO	TIGR02529 (HMM): ethanolamine utilization protein EutJ family protein	GO:0046336: ethanolamine catabolic process
microcompartment coat protein	eutM	YES	PF00936 (HMM-CLUST): BMC domain	GO:0046336: ethanolamine catabolic process
carboxysome coat protein homolog	eutN	YES	PF03319 (HMM-CLUST): ethanolamine utilisation protein EutN/carboxysome	GO:0046336: ethanolamine catabolic process
ethanolamine utilization protein, EutP	eutP	NO	TIGR02528 (HMM-CLUST): ethanolamine utilization protein, EutP	GO:0046336: ethanolamine catabolic process
ethanolamine utilization protein, PduL homolog	eutPduL	NO	PF06130 (HMM-CLUST): propanediol utilization protein PduL	GO:0046336: ethanolamine catabolic process
carboxysome coat protein homolog	eutPduT	NO	TIGR02526 (HMM): PduT-like ethanolamine utilization protein	GO:0046336: ethanolamine catabolic process
ethanolamine utilization protein, EutQ	eutQ	NO	PF06249 (HMM-CLUST): ethanolamine utilization protein EutQ	GO:0046336: ethanolamine catabolic process
cob(I)alamin adenosyltransferase	eutT	NO	PF01923 (HMM-CLUST): putative ATP:cob(I)alamin adenosyltransferase	GO:0046336: ethanolamine catabolic process
regulatory components	regul	NO	PF00007 (HMM-CLUST): cystine-knot domain PF07568 (HMM-CLUST): histidine kinase PF00072 (HMM-CLUST): response regulator receiver domain PF00165 (HMM-CLUST): transcriptional regulator, AraC family	GO:0046336: ethanolamine catabolic process GO:0046336: ethanolamine catabolic process GO:0046336: ethanolamine catabolic process GO:0046336: ethanolamine catabolic process

Parent Properties

Accession	Name
GenProp0294	ethanolamine degradation proteinaceous organelle