Genome Property Definition Page

Accession

GenProp0265

Name

trehalose biosynthesis, OtsAB pathway

Type

PATHWAY

Description

Trehalose is compatible solute, an osmolyte that protects cells against osmotic stress. It is built from two glucose molecules in an alpha,alpha linkage and can be made by at least three different pathways. In the OtsAB pathway (ots stands for osmotically regulated trehalose synthesis), OtsA (EC 2.4.1.15) makes trehalose-6-phosphate from UDP-glucose and D-glucose 6-phosphate. OtsB (EC 3.1.3.12) removes the phosphate.

JCVI Role

Adaptations to atypical conditions

Parent Property

GenProp0267: osmolyte biosynthesis

Literature References

[ 1 ]Wolf A, Kramer R, Morbach S Three pathways for trehalose metabolism in Corynebacterium glutamicum ATCC13032 and their significance in response to osmotic stress. Mol Microbiol 2003 Aug;49(4):1119-34. PMID 12890033
[ 2 ]Kaasen I, McDougall J, Strom AR Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex. Gene 1994 Jul 22;145(1):9-15. PMID 8045430

Gene Ontology Term

GO:0005992: trehalose biosynthetic process (biological_process)
GO:0006970: response to osmotic stress (biological_process)

Components

Step Name	Step Num	Required	Evidence (Method)	Evidence Go Terms
Trehalose-6-phosphate synthase	otsA	YES	TIGR02400 (HMM): alpha,alpha-trehalose-phosphate synthase (UDP-forming)	GO:0005992: trehalose biosynthetic process GO:0006970: response to osmotic stress
trehalose-phosphatase	otsB	YES	TIGR00685 (HMM): trehalose-phosphatase	GO:0005992: trehalose biosynthetic process GO:0006970: response to osmotic stress

Parent Properties

Accession	Name
GenProp0267	osmolyte biosynthesis

Sibling Properties

Accession	Name
GenProp0147	glycine betaine biosynthesis from choline
GenProp0264	glucosylglycerol biosynthesis
GenProp0266	trehalose biosynthesis, TreYZ pathway
GenProp0268	ectoine biosynthesis
GenProp0281	mannosylglycerate biosynthesis
GenProp0878	N(epsilon)-acetyl-beta-lysine biosynthesis
GenProp1070	beta-glutamate biosynthesis