Genome Property Definition Page

Namethiotemplate type non-ribosomal peptide biosynthesis
DescriptionNon-ribosomal peptides are an important class of natural product which are assembled by the sequential addition of units containing amino acid groups in peptide linkages. These natural products include antibiotics, siderophores, toxins and other compounds of biological and economic importance. These products are synthesized on large proteins called non-ribosomal peptide synthetases (NRPSs) which specify the amino acids to be added by the sequence of amino acid adenylation (activation) domains along the synthetase. NRPSs may utilize many more amino acids than the 20 to which the ribosomes are limited. In addition, NRPSs may form linkages between side-chain amino and carboxylate groups and may contain domains which catalyze epimerization, methylation, cross-linking, cyclization, oxidation, reduction and other modifications to the growing peptide chain. Chain initiation may incorporate fatty acids (in the case of lipodepsipeptides) or other non-amino acid, carboxylate-containing compounds and NRPSs may work in concert with polyketide synthetases to form hybrid peptide-ketide natural products. NRPSs, like type I polyketide synthetases, work via a thio-template mechanism in which the activated units are transferred to the thiol of bound pantetheine groups in a thio-ester linkage (of the amino acid carboxylates). Condensation domains then join two thioester-bound units to form a peptide bond. Release of the complete polypeptide chain may be via cyclization or through the action of a thioesterase or thioester reductase. Many of the constituent domains of NRPSs have been defined by HMM models. These include adenylation (AMP-binding enzyme, PF00501), condensation (PF00668), acyl/peptidyl carrier protein (thiolation, pantetheine-binding, PF00550), thioesterase (PF00975) and thioester reductase (TIGR01746) domains. In addition, the pantetheine binding domains are identified by a pair of PROSITE motifs (PS00012, PS50075). The minimal components of an NRPS are the C (condensation), A (adenylation) and T (thiolation) domains which are commonly organized into CAT modules. NRPSs are detected as genes which contain hits to all three of these domains. Genomes with at least one ORF which fulfills this condition will be assigned the YES state for this property. The value field will hold the number of ORFs found to be NRPSs. NRPSs may, however, be broken up into multiple genes at domain boundaries and thus the possibility exists of missing NRPS genes which are short and broken up into pieces having only two of the three essential domains. If the state NO has been set, this indicates that the genome has been manually reviewed.
Parent PropertyGenProp0077: natural products biosynthesis
Gene Ontology TermGO:0019184: nonribosomal peptide biosynthetic process (biological_process)

Step NameStep NumRequiredEvidence (Method)Evidence Go Terms
condensation, adenylation and thiloation domains in a single ORFC+A+TYESC+A+T dom (RULES)
GO:0019184: nonribosomal peptide biosynthetic process

Parent Properties
GenProp0077natural products biosynthesis

Sibling Properties
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GenProp0015polyketide biosynthesis, type II
GenProp0169hybrid NRPS-PKS natural pruduct biosynthesis genes
GenProp0724phosphonoacetaldehyde biosynthesis from phosphoenolpyruvate
GenProp0757quorum-sensing, autoinducer-2 system
GenProp0758lycopene biosynthesis from IPP
GenProp0770siderophore biosynthesis
GenProp0807bacteriocin system, heterocycle biosynthesis group
GenProp0808bacteriocin system, TIGR01847 leader group
GenProp0809bacteriocin system, lactococcin 972 group
GenProp0823violacein biosynthesis
GenProp0825bacteriocin system, circular bacteriocin group
GenProp0853lantibiotic system, gallidermin/epidermin family
GenProp0861bacteriocin system, NHLP (nif11/nitrile hydratase leader peptide) transport group
GenProp0901post-ribosomal natural product synthesis system, Burkholderia TOMM-type
GenProp0919SCIFF/radical SAM Clostridial gene pair
GenProp0920radical SAM Y_X(10)_GDL system
GenProp0921radical SAM pair and His-Xaa-Ser repeats peptide
GenProp0936bacteriocin system, sporulation delay protein group
GenProp0945radical SAM maturase bacteriocin system, CLI_3235 type
GenProp0954radical SAM-cylized peptide, Pep1357C family
GenProp0955modified peptide/radical SAM maturase system, YydFG family
GenProp0956radical SAM maturase/selenobacteriocin system
GenProp0962methanobactin biosynthesis, Mb-OB3b family
GenProp0967radical SAM maturase system, CXXX repeats type
GenProp0982radical SAM maturase system, FibroRumin system
GenProp0984radical SAM maturase system, GG-Bacteroidales group
GenProp0990cobaltopeptin biosynthesis
GenProp0991ATP-grasp maturase system, microviridin/marinostatin class
GenProp1000ATP-grasp maturase system, uncharacterized
GenProp1002grasp-with-spasm peptide maturase system
GenProp1003cysteine S-glycopeptide biosynthesis, sublancin family
GenProp1029radical SAM/SPASM system SynChlorMet
GenProp1037radical SAM/SPASM system GRRM
GenProp1052geopeptide radical SAM/SPASM maturase system
GenProp1062radical SAM/SPASM system Clo7bot
GenProp1065radical SAM/SPASM TIGR04347/TIGR04031 system
GenProp1078sporulation killing factor system
GenProp1083cyanobactin-like ribosomal natural product biosynthesis
GenProp1084enduracididine biosynthesis
GenProp1090radical SAM/SPASM maturase system XYE
GenProp1097bacimethrin biosynthesis
GenProp10983-methylarginine biosynthesis